Journal: BMC Biology

Article Title: Spatial-temporal targeting of lung-specific mesenchyme by a Tbx4 enhancer

doi: 10.1186/1741-7007-11-111

Figure Lengend Snippet: Differential targeting of lung vascular endothelial cells by the Tbx4 lung enhancer at different gestational ages. (A) In E18.5 lung of the triple transgenic mice with Dox induction from E6.5, PECAM-1-positive endothelial cells were GFP positive, but LacZ negative. (B-C) in the E15.5 lung of the triple transgenic mice with different Dox induction time windows as indicated above the panel, vascular endothelial cells were GFP-positive if Dox induction was given prior to E10.5 (B) , but LacZ expression was already negative. Consistently, Dox induction from E11.5 to E15.5 was not able to target vascular endothelial cells, shown by double negative staining for GFP and LacZ (C) . V: vasculature. Dox, doxycycline; E, embryonic day; PECAM-1, platelet endothelial cell adhesion molecule.

Article Snippet: The related antibodies were: rabbit anti-GFP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-GFP (Abcam, Cambridge, MA, USA), mouse anti-cytokeratin and mouse anti-SMA (Sigma), rabbit anti-PECAM-1 (LSBio, Seattle, WA, USA), rabbit anti-lacZ (MP Biomedicals, Solon, OH, USA), mouse anti-ADRP (BioGenex, Fremont, CA, USA), rabbit anti-NG2 chondroitin sulfate proteoglycan (Millipore, Billerica, MA, USA), hamster anti-T1α (DSHB at the University of Iowa) and mouse anti-CGRP (Sigma).

Techniques: Transgenic Assay, Expressing, Negative Staining

Journal: BMC Biology

Article Title: Spatial-temporal targeting of lung-specific mesenchyme by a Tbx4 enhancer

doi: 10.1186/1741-7007-11-111

Figure Lengend Snippet: NG2-positive cells were targeted by the Tbx4 -lung enhancer driven Tet-On system. Lung tissue sections of the triple transgenic mice (Tbx4-rtTA/TetO-Cre/mT-mG) with different Dox induction as indicated were co-immunostained by GFP, NG2, and SMA or PECAM-1. (A) In the E18.5 lung of the triple transgenic mice with Dox induction from E6.5 to E18.5, NG2-positive cells, including SMA-positive vasculature smooth muscle cells and SMA-negative pericytes, were all marked by GFP expression, suggesting that these cells were targeted by the Tet-On system in fetuses. (B-C) With Dox induction at either mid-gestation (E11.5 to E15.5) or late gestation (E15.5 to E18.5), most NG-2 positive cells of the triple transgenic mouse lungs at E15.5 (B) or E18.5 (C) were positive for GFP. These NG2-positive cells were adjacent to PECAM-1 positive endothelial cells. Dox, doxycycline; E, embryonic day; PECAM-1, platelet endothelial cell adhesion molecule; SMA, α-smooth muscle actin.

Article Snippet: The related antibodies were: rabbit anti-GFP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-GFP (Abcam, Cambridge, MA, USA), mouse anti-cytokeratin and mouse anti-SMA (Sigma), rabbit anti-PECAM-1 (LSBio, Seattle, WA, USA), rabbit anti-lacZ (MP Biomedicals, Solon, OH, USA), mouse anti-ADRP (BioGenex, Fremont, CA, USA), rabbit anti-NG2 chondroitin sulfate proteoglycan (Millipore, Billerica, MA, USA), hamster anti-T1α (DSHB at the University of Iowa) and mouse anti-CGRP (Sigma).

Techniques: Transgenic Assay, Expressing